MGF experiences/cycle protocols?

[IRON37]

Hey guys, just wondering if anyone here has ever actually used MGF before(by itself or along with LR3 IGF)? I cant really find anyone on the boards that can say they have. Just alot of theories etc. And I can recall a mod saying MGF could be dangerous in a way. How is that? Im sure those are alot of basic questions but I cant really find too much info on it yet.

[liftsiron]

My Pegylated Mechano Growth Factor trial

By Gavin Kane (2006)
Almost Pro
proffesionalmuslce.com

I was originally going to wait until the end of my trail with PEG/MGF (herein further referred to as pmgf) before posting my results and information, but my PM box has been flooded with information requests, so instead of waiting, I am going to post a blog that will be updated weekly.

Let’s start with an explanation of Mechano Growth Factor (MGF) and what it does. The C-terminal peptide of mechano-growth factor (MGF), an alternatively spliced variant of insulin-like growth factor 1 (IGF-1), was found to function independently from the rest of the molecule. At any rate, IGF-I exists in multiple isoforms (tissue-specific proteins of functional and structural similarity). One isoform, which differs from the systemic or liver type, happens to be particularly sensitive to mechanical signals such as the gamut of exercise overload. This important isoform, called mechano-growth factor (MGF), is a local splice variant of IGF-I produced by damaged or loaded skeletal muscle.

The physiological function of a recently cloned splice variant of insulin-like growth factor-I (IGF-I; mechano growth factor (MGF)) was studied using an in vitro cell model. Unlike mature IGF-I, the distinct E domain of MGF inhibits terminal differentiation whilst increasing myoblast proliferation. Blocking the IGF-I receptor with a specific antibody indicated that the function of MGF E domain is mediated via a different receptor. The results provide a basis for localized tissue adaptation and helps explain why loss of muscle mass occurs in the elderly and in dystrophic muscle in which MGF production is markedly affected.
Yang SY, Goldspink G. FEBS Lett. 2002 Jul 3;522(1-3):156-60.

So what is pegylation? In simple terms it is the process of attaching one or more chains of a substance called polyethylene glycol (PEG) to a protein molecule such as IGF or in this case MGF. Since the body does not react to PEG, it helps provide a protective barrier around an attached protein so it can survive in the body longer. This is highly beneficial for systemic products that must survive repeated attacks by enzymatic exposure. PEG is an inert non-toxic substance that can provide protection to amine groups since they are flexible and allow attachment by bioengineered processes to the receptor bearing cell.

Finally a quick explanation of polyethylene glycol; Any of a family of high molecular weight compounds that can be liquid or waxlike in consistency and are soluble in water and in many organic solvents.

Alright, now that you have sifted through my semi-scientific yet easy to read explanation of pmeg, let’s get to the main point of the article, my experience. First off, I was asked to test this new product by IBE. They supplied me the product and requested I use it at 200mcg, two times per week. I agreed on the condition that no matter what the results, good or bad, my review would be brutally honest. If they were looking for someone to just promote their product, I was not their guy. They agreed unconditionally and shipped the product out immediately.

Let me preface what follows below are my results but they will not be typical for most users. Having already accumulated up to 270lbs of mass over the years, my muscle memory is outstanding and I am easily able to rebound when using products. At this time, I have been off cycle for 2 months, using only 500mg test enanthate per week as HRT. This is my first time off cycle in over 8 years. I was also using 2iu gh per day but stopped using it at the request of IBE. My diet and training did not change, I was trying to create as stable an environment to test this product as would allow.

I started at a current bodyweight of 227lbs with my bodyfat measured at 14.8% and 55% water balance. I have some sophisticated test equipment at home so I can be sure my stats and my ability to track results are fairly accurate. I have been using this product for 4 weeks now, still having 4 weeks to go. After that, I will cycle off for a few weeks and then resume with a new test trial using mgf, igf, slin, and gh with small amount of anabolics in a very androgen rich environment. I will utilize many small injections with specific timing to maximize my results, but that is for another article.

My first week of use was a tough one for me, I had to come off the gh and immediately notice a decrease in lean mass, as well as longer recovery between workouts. This subsided very quickly though with the introduction of pmeg. My current workout schedule is on Tues, Thurs, and Friday. I decided to use the pmeg on Tues and Thurs, my two biggest bodypart workout days. I was instructed by IBE to test it sub-q since it is systemic anyway and the injection method was somewhat irrelevant. On my next trial, I will be using it IM in a specific sequence with other peptides.

My first week of use, my weight stabilized as did my body fat percentage. I was quickly able to stop my wasting from coming off cycle and gh, and reintroduce beneficial cell growth potential. The changes from my first to second week were dramatic to say the least. Not only was I getting skin tight pumps, my vascularity was up, but the scale was most telling off all. I went from 227lbs to 232lbs by the end of the second week. At this time my water balance and body fat were still the same. The third week was another major change, even more so than the first two weeks. My weight jumped up to 235lbs but most incredible of all was the fat loss. Let me remind you that I did not make any diet changes; I just added this product in mostly as a pct product while coming off cycle. So what impressed my most was my body fat dropping from 14.8% to 12.9%. I just finished my 4th week yesterday so I jumped back on the scale to see what changes were in store for me now!

This was the best week yet. Before you get all freaked out, please remember that I just came off an 8 year cycle, my body fat jumped up from the estrogen increase and I was gaining back weight I had lost from not bulking anymore. Ok, with that out of the way again, here is week 4: Weight backed off a bit to 234lbs, but my body fat melted down to 10.9%, a drop of almost 4% since starting this just 4 weeks ago. The other awesome gain was my cellular water balance was up to 58% which is an indication of intra-cellular water balance for overall health, condition, and prevention from muscle and joint tissue damage.

Alright, that is all for now, so stop PM’ing me, lol. I will update this at the end of each week so you guys can follow along and check on my updates.

[Deacon]

I have read a study where they even suggested that MGF is so effective alone you dont need IGF-1 with it - in fact they say it works better alone

[Aber1784]

I have read a study where they even suggested that MGF is so effective alone you dont need IGF-1 with it - in fact they say it works better alone

could you post the study or the link for it? im about to give these both a try soon and would like to read the study....

[Deacon]

it was in MD magazine bro - a big article on MGF

[psimonkey]

I did post this elsewhere so excuse me for the repetition.

https://www.mesomorphosis.com/steroid-profiles/mechano-growth-factor.htm

by Anthony Roberts - After resistance exercise, IGF-1 is released within the muscle. Specifically, at this time, immediately following the mechanical use of a muscle, the IGF-I gene is spliced towards MGF which initiates hypertrophy and repair of local muscle damage. It does so both by activating muscle stem cells and satellite cells, but also via various other anabolic processes. (1) It differs from “regular” IGF-1 mainly due to it’s C-Terminal sequence.

Background

At the time of this writing, bodybuilders and athletes have been experimenting with MGF for a couple of years already. It was first discovered in the muscle by Goldspink, et al. In human muscle, a 49-base insert changes the reading frame in mechano growth factor (MGF) as compared to IGF-1.

Action

When mechanical overload is introduced to a muscle (as by weight training), the IGF-1 gene released and is differentially spliced during the bodies response. Initially, it it is spliced to produce predominantly IGF-1Ec (called the MGF splice variant of IGF-1). This early splicing stimulates satellite cells into activation. Which in turn allows the activation of extra undamaged nuclei to grow new muscle fiber and tissue. The appearance of MGF also initiates the upregulation of new protein synthesis. After this initial splicing of IGF-1 into MGF, production then switches towards producing a systemic release of IGF-1Ea from the liver, which also upregulates protein synthesis as well. The expression of IGF-1 splice variants, over the course of the healing and regrowth phase of muscle repair is thought to be the primary anabolic mechanism by which the body produces new muscle. MGF is available as an injectable peptide, and it has been anecdotally shown that injecting it will cause a response in the area resulting in localized muscle growth.

Technical Data

In a rodent study, a single intramuscular injection into muscle resulted in a 25% increase in mean muscle fibre cross section area within three weeks.(2) Using a similar protocol, liver-derived IGF-1 took four months to produce a 15% increase.(3)

It would also appear that with regards to age, the young have a better ability to respond to MGF (4), and that the elderly experience a decreased response to MGF which results in a decreased ability to stimulate the growth of new muscle tissue.

In the words of the man known as the father of MGF research:

“MGF is… a prime candidate for gene doping for enhancement of athletic performance.”(1)

User Notes

I’ve personally used MGF several times and think it’s a really great product. Admittedly, I have only used it in conjunction with Lr3IGF-1, but many (myself included) feel that there’s probably quite a bit of synergy to be found between these two peptides.

I experienced muscle growth (localized) with MGF, but not really much of a strength increase over what I would have experienced with just the IGF. Most users find MGF to be very good for muscle growth and typically use a protocol of 100mcgs injected into each lagging muscle just after working it.

A further discussion of how to incorporate this compound can be found in my article “Peptides: The next frontier in hypertrophy”

Trivial Name - Mechano Growth Factor
Systematic Name - †IGF-1Ec
Bioavailability - 99%
Metabolism - Almost all tissues
Elimination Half Life - Variable
Excretion - N/A
Legal Status - Varies
Route of Administration - Subcutaneous injection, Intramuscular Injection

References

1. British Journal of Sports Medicine 2005;39:787-788; doi:10.1136/bjsm.2004.015826 © 2005 by BMJ Publishing Group Ltd & British Association of Sport and Exercise Medicine
2. Goldspink G, Yang SY. Method of treating muscular disorders. United States Patent. Patent No US 6,221,842 B1, Apr 24 2001.
3. Barton-Davis E, Shoturma DI, Musaro A, et al. Viral mediated expression of insulin-like growth factor-I blocks the aging-related loss of skeletal muscle function. Proc Natl Acad Sci USA 1998;95:15603–7
4. J Physiol (2003), 547.1, pp. 247-254© Copyright 2003 The Physiological SocietyDOI: 10.1113/jphysiol.2002.032136

[psimonkey]

Please expand DRveejay11 How did you use it? What body parts? What training routine? I have never used MGF so I am very interested. Thanks.